JARID2 monoclonal antibody, clone 7H1B3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) JARID2-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with JARID2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JARID2 monoclonal antibody, clone 7H1B3.
Flow Cytometry
Flow cytometric analysis of HeLa cells with JARID2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human JARID2.
Immunogen
Recombinant protein corresponding to amino acid 1097-1246 of human JARID2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
139kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) JARID2-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with JARID2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JARID2 monoclonal antibody, clone 7H1B3.Flow Cytometry
Flow cytometric analysis of HeLa cells with JARID2 monoclonal antibody (green) and negative control (red). -
Gene Info — JARID2
Entrez GeneID
3720Gene Name
JARID2
Gene Alias
JMJ
Gene Description
jumonji, AT rich interactive domain 2
Omim ID
601594Gene Ontology
HyperlinkGene Summary
This gene is an ortholog of the mouse jumonji gene, which encodes a nuclear protein essential for mouse embryogenesis, including neural tube formation. Overexpression of mouse jumonji negatively regulates cell proliferation. The jumonji proteins contain a DNA-binding domain, called an AT-rich interaction domain (ARID), and share regions of similarity with human retinoblastoma-binding protein-2 and the human SMCX protein. [provided by RefSeq
Other Designations
OTTHUMP00000016058|jumonji homolog|jumonji, AT rich interactive domain 2 protein|jumonji-like protein
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Interactome
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Disease
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