KPNA2 monoclonal antibody, clone 1E8B7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: HEK293 cell; Lane 3: NIH/3T3 cell with KPNA2 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) KPNA2-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with KPNA2 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with KPNA2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of KPNA2 monoclonal antibody, clone 1E8B7.
Flow Cytometry
Flow cytometric analysis of HeLa cells with KPNA2 monoclonal antibody (green) and negative control (red)
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human KPNA2.
Immunogen
Recombinant protein corresponding to amino acid 1-530 of human KPNA2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
58kDa
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: HEK293 cell; Lane 3: NIH/3T3 cell with KPNA2 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) KPNA2-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with KPNA2 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with KPNA2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of KPNA2 monoclonal antibody, clone 1E8B7.Flow Cytometry
Flow cytometric analysis of HeLa cells with KPNA2 monoclonal antibody (green) and negative control (red) -
Gene Info — KPNA2
Entrez GeneID
3838Gene Name
KPNA2
Gene Alias
IPOA1, QIP2, RCH1, SRP1alpha
Gene Description
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Omim ID
600685Gene Ontology
HyperlinkGene Summary
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq
Other Designations
RAG cohort 1|importin alpha 1|importin alpha 2|importin-alpha-P1|karyopherin alpha 2|pendulin
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Interactome
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Disease
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