Nlrc4 (phospho S533) monoclonal antibody, clone 9A6A1
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Specification
Product Description
Rat monoclonal antibody raised against synthetic peptide of mouse Nlrc4 (phospho S533).
Immunogen
A synthetic peptide corresponding to amino acid 525-538 of human Nlrc4 (phospho S533).
Host
Rat
Theoretical MW (kDa)
116.7kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunocytochemical staining of HeLa cells with Nlrc4 (phospho S533) monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Nlrc4 (phospho S533) monoclonal antibody, clone 9A6A1Flow Cytometry
Flow cytometric analysis of HeLa cells with Nlrc4 (phospho S533) monoclonal antibody (green) and negative control (red). -
Gene Info — Nlrc4
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