Nlrc4 (phospho S533) monoclonal antibody, clone 4B7B7
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Specification
Product Description
Rat monoclonal antibody raised against synthetic peptide of mouse Nlrc4 (phospho S533).
Immunogen
A synthetic peptide corresponding to amino acid 525-538 of human Nlrc4 (phospho S533).
Host
Rat
Theoretical MW (kDa)
116.7kDa
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using Nlrc4 (phospho S533) monoclonal antibody against NIH/3T3 cell.Immunocytochemistry
Immunocytochemical staining of HeLa cells with Nlrc4 (phospho S533) monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Nlrc4 (phospho S533) monoclonal antibody, clone 4B7B7. -
Gene Info — Nlrc4
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