ATG13 monoclonal antibody, clone 3E4C8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ATG13-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ATG13 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG13 monoclonal antibody, clone 3E4C8.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ATG13 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ATG13.
Immunogen
Recombinant protein corresponding to amino acid 339-550 of human ATG13 from E. coli.
Host
Mouse
Theoretical MW (kDa)
56.6kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ATG13-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ATG13 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG13 monoclonal antibody, clone 3E4C8.Flow Cytometry
Flow cytometric analysis of HeLa cells with ATG13 monoclonal antibody (green) and negative control (red). -
Gene Info — ATG13
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Interactome
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