PRDM5 monoclonal antibody, clone 7D4C12
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PRDM5.
Immunogen
Recombinant protein corresponding to amino acid 1-100 of human PRDM5 from E. coli.
Host
Mouse
Theoretical MW (kDa)
73kDa
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HL-60 cell; Lane 2: NIH/3T3 cell; Lane 3: SW480 cell; Lane 4: HEK293 cell with PRDM5 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PRDM5-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with PRDM5 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PRDM5 monoclonal antibody, clone 7D4C12.Flow Cytometry
Flow cytometric analysis of HeLa cells with PRDM5 monoclonal antibody (green) and negative control (red). -
Gene Info — PRDM5
Entrez GeneID
11107Gene Name
PRDM5
Gene Alias
PFM2
Gene Description
PR domain containing 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. [provided by RefSeq
Other Designations
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Interactome
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Disease
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