WNT3A monoclonal antibody, clone 1E6G4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell; Lane 2: HepG2 cell; Lane 3: A431 cell with WNT3A monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) WNT3A-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectum cancer tissues with WNT3A monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with WNT3A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WNT3A monoclonal antibody, clone 1E6G4.
Flow Cytometry
Flow cytometric analysis of HeLa cells with WNT3A monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human WNT3A.
Immunogen
Recombinant protein corresponding to amino acid 170-352 of human WNT3A from E. coli.
Host
Mouse
Theoretical MW (kDa)
39.4kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell; Lane 2: HepG2 cell; Lane 3: A431 cell with WNT3A monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) WNT3A-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectum cancer tissues with WNT3A monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with WNT3A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WNT3A monoclonal antibody, clone 1E6G4.Flow Cytometry
Flow cytometric analysis of HeLa cells with WNT3A monoclonal antibody (green) and negative control (red). -
Gene Info — WNT3A
Entrez GeneID
89780Gene Name
WNT3A
Gene Alias
MGC119418, MGC119419, MGC119420
Gene Description
wingless-type MMTV integration site family, member 3A
Omim ID
606359Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq
Other Designations
OTTHUMP00000035713
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Interactome
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Pathway
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Disease
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