SIRT4 monoclonal antibody, clone 6A12D7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) SIRT4-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with SIRT4 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SIRT4 monoclonal antibody, clone 6A12D7.
Flow Cytometry
Flow cytometric analysis of HeLa cells with SIRT4 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SIRT4.
Immunogen
Recombinant protein corresponding to amino acid 215-314 of human SIRT4 from E. coli.
Host
Mouse
Theoretical MW (kDa)
35.2kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) SIRT4-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with SIRT4 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SIRT4 monoclonal antibody, clone 6A12D7.Flow Cytometry
Flow cytometric analysis of HeLa cells with SIRT4 monoclonal antibody (green) and negative control (red). -
Gene Info — SIRT4
Entrez GeneID
23409Gene Name
SIRT4
Gene Alias
MGC130046, MGC130047, MGC57437, SIR2L4
Gene Description
sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Omim ID
604482Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq
Other Designations
sir2-like 4|sirtuin 4|sirtuin type 4
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Interactome
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Disease
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