HIST2H4A(20Me) monoclonal antibody, clone 3E7D9
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with HIST2H4A(20Me) monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with HIST2H4A(20Me) monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIST2H4A(20Me) monoclonal antibody, clone 3E7D9.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human HIST2H4A(20Me).
Immunogen
A synthetic peptide corresponding to amino acid of human HIST2H4A(20Me).
Host
Mouse
Theoretical MW (kDa)
11.4kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with HIST2H4A(20Me) monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with HIST2H4A(20Me) monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIST2H4A(20Me) monoclonal antibody, clone 3E7D9. -
Gene Info — HIST2H4A
Entrez GeneID
8370Gene Name
HIST2H4A
Gene Alias
FO108, H4, H4/n, H4F2, H4FN, HIST2H4
Gene Description
histone cluster 2, H4a
Omim ID
142750Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq
Other Designations
H4 histone family, member N|H4 histone, family 2|OTTHUMP00000013906|OTTHUMP00000194862|OTTHUMP00000194863|histone 2, H4a|histone IV, family 2
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