Ffar1 monoclonal antibody, clone G16
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Immunofluorescence analysis of HEK 293T cells overexpressing human FFAR1, using Ffar1 monoclonal antibody, clone G16 (Cat # MAB1714) .
-
Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Ffar1.
Immunogen
A synthetic peptide corresponding to mouse Ffar1.
Host
Mouse
Reactivity
Mouse
Form
Liquid
Preparation Method
The splenic lymphocytes from GANP mouse, immunized with the first extracellular domain peptides of mouse Ffar1 conjugated with KLH, were fused to myeloma P3U1 cells. The screening of the hybridoma cells was performed on ELISA. The cell line with positive reaction was grown on non-serum medium, from which the antibody was purified by Protein G affinity chromatography.
Purification
Protein G affinity chromatography
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Flow Cytometry (0.5 ug/mL)
Immunocytochemistry (1 ug/mL)
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% proclin, 2.0% Block Ace).
Storage Instruction
Store at below -20°C. Once thawed, store at 4°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HEK 293T cells overexpressing human FFAR1, using Ffar1 monoclonal antibody, clone G16 (Cat # MAB1714) .Flow Cytometry
-
Gene Info — Ffar1
-
Publication Reference
-
Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1).
Hirasawa A, Itsubo C, Sadakane K, Hara T, Shinagawa S, Koga H, Nose H, Koshimizu TA, Tsujimoto G.
Biochemical and Biophysical Research Communications 2008 Jan; 365(1):22.
Application：Flow Cyt, IF, IHC, IP , Human, Mouse, HEK 293 cells, Human PBMCs, Mouse spleen.
-
The FFA receptor GPR40 links hyperinsulinemia, hepatic steatosis, and impaired glucose homeostasis in mouse.
Steneberg P, Rubins N, Bartoov-Shifman R, Walker MD, Edlund H.
Cell Metabolism 2005 Apr; 1(4):245.
-
Oleate promotes the proliferation of breast cancer cells via the G protein-coupled receptor GPR40.
Hardy S, St-Onge GG, Joly E, Langelier Y, Prentki M.
The Journal of Biological Chemistry 2005 Apr; 280(14):13285.
-
Free fatty acids regulate insulin secretion from pancreatic beta cells through GPR40.
Itoh Y, Kawamata Y, Harada M, Kobayashi M, Fujii R, Fukusumi S, Ogi K, Hosoya M, Tanaka Y, Uejima H, Tanaka H, Maruyama M, Satoh R, Okubo S, Kizawa H, Komatsu H, Matsumura F, Noguchi Y, Shinohara T, Hinuma S, Fujisawa Y, Fujino M.
Nature 2003 Mar; 422(6928):173.
-
The orphan G protein-coupled receptor GPR40 is activated by medium and long chain fatty acids.
Briscoe CP, Tadayyon M, Andrews JL, Benson WG, Chambers JK, Eilert MM, Ellis C, Elshourbagy NA, Goetz AS, Minnick DT, Murdock PR, Sauls HR Jr, Shabon U, Spinage LD, Strum JC, Szekeres PG, Tan KB, Way JM, Ignar DM, Wilson S, Muir AI.
The Journal of Biological Chemistry 2002 Mar; 278(13):11303.
-
Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1).
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com