BAT1 monoclonal antibody, clone 2F5G7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: HepG2 cell; Lane 3: K562 cell; Lane 4: Jurkat cell; Lane 5: NIH/3T3 cell; Lane 6: MCF-7 cell; Lane 7: Jurkat cell; Lane 8: Hek293 cell with BAT1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) BAT1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded HeLa tissues with BAT1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with BAT1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BAT1 monoclonal antibody, clone 2F5G7.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BAT1.
Immunogen
Recombinant protein corresponding to amino acid 1-250 of human BAT1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
49kDa
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: HepG2 cell; Lane 3: K562 cell; Lane 4: Jurkat cell; Lane 5: NIH/3T3 cell; Lane 6: MCF-7 cell; Lane 7: Jurkat cell; Lane 8: Hek293 cell with BAT1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) BAT1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded HeLa tissues with BAT1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with BAT1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BAT1 monoclonal antibody, clone 2F5G7. -
Gene Info — BAT1
Entrez GeneID
7919Gene Name
BAT1
Gene Alias
D6S81E, DDX39B, UAP56
Gene Description
HLA-B associated transcript 1
Omim ID
142560Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq
Other Designations
56 kDa U2AF65-associated protein|ATP-dependent RNA helicase p47|DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B|DEAD-box protein UAP56|OTTHUMP00000029228|OTTHUMP00000029229|OTTHUMP00000035591|OTTHUMP00000035963|OTTHUMP00000035964|OTTHUMP00000035965|OTTHUMP0000
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Interactome
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Disease
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