NOS1 monoclonal antibody, clone 2E11G6
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NOS1.
Immunogen
Recombinant protein corresponding to amino acid 17-153 of human NOS1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
161kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) NOS1-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with NOS1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NOS1 monoclonal antibody, clone 2E11G6.Flow Cytometry
Flow cytometric analysis of HeLa cells with NOS1 monoclonal antibody (green) and negative control (red). -
Gene Info — NOS1
Entrez GeneID
4842Gene Name
NOS1
Gene Alias
IHPS1, NOS, nNOS
Gene Description
nitric oxide synthase 1 (neuronal)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. This gene encodes a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in this gene are associated with infantile hypertrophic pyloric stenosis type 1
Other Designations
neuronal nitric oxide synthase
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