PPARA monoclonal antibody, clone 5D10C3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: HEK293 cell; Lane 3: COS-7 cell; Lane 4: HepG2 cell; Lane 5: Jurkat cell with PPARA monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PPARA-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PPARA monoclonal antibody, clone 5D10C3.
Flow Cytometry
Flow cytometric analysis of Jurkat cells with PPARA monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PPARA.
Immunogen
Recombinant protein corresponding to amino acid 1-120 of human PPARA from E. coli.
Host
Mouse
Theoretical MW (kDa)
52.2kDa
Reactivity
Human, Monkey
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: HEK293 cell; Lane 3: COS-7 cell; Lane 4: HepG2 cell; Lane 5: Jurkat cell with PPARA monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PPARA-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PPARA monoclonal antibody, clone 5D10C3.Flow Cytometry
Flow cytometric analysis of Jurkat cells with PPARA monoclonal antibody (green) and negative control (red). -
Gene Info — PPARA
Entrez GeneID
5465Gene Name
PPARA
Gene Alias
MGC2237, MGC2452, NR1C1, PPAR, hPPAR
Gene Description
peroxisome proliferator-activated receptor alpha
Omim ID
170998Gene Ontology
HyperlinkGene Summary
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq
Other Designations
OTTHUMP00000028713|OTTHUMP00000042872|peroxisome proliferative activated receptor, alpha|peroxisome proliferator activated receptor alpha
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Interactome
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Pathway
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Disease
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