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Last updated: 2020/7/5

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PPARA monoclonal antibody, clone 5D10E10 

  • Catalog # : MAB17127
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human PPARA.
  • Immunogen:
  • Recombinant protein corresponding to amino acid 1-120 of human PPARA from E. coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 52.2kDa
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry
    Immunocytochemistry
    Flow Cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of (1) HEK293 cells, (2) PPARA-hIgGFc transfected HEK293 cell lysate.
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis of PPARA monoclonal antibody, clone 5D10E10.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells with PPARA monoclonal antibody (green) and negative control (red).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5465
  • Gene Name:
  • PPARA
  • Gene Alias:
  • MGC2237,MGC2452,NR1C1,PPAR,hPPAR
  • Gene Description:
  • peroxisome proliferator-activated receptor alpha
  • Gene Summary:
  • Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000028713,OTTHUMP00000042872,peroxisome proliferative activated receptor, alpha,peroxisome proliferator activated receptor alpha
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