ATG16L1 monoclonal antibody, clone 5H9A11
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More Files
- More Functions
Western Blot (Cell lysate)
Westerb blot analysis of Lane 1: HeLa cell; Lane 2: Raji cell; Lane 3: PANC-1 cell; Lane 4: Jurkat cell; Lane 5: PC-12 cell; Lane 6: HepG2 cell; Lane 7: Hek293 cell; Lane 8: NIH3T3 cell with ATG16L1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ATG16L1-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG16L1 monoclonal antibody, clone 5H9A11.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ATG16L1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ATG16L1.
Immunogen
Recombinant protein corresponding to amino acid 11-257 of human ATG16L1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
68.3kDa
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Westerb blot analysis of Lane 1: HeLa cell; Lane 2: Raji cell; Lane 3: PANC-1 cell; Lane 4: Jurkat cell; Lane 5: PC-12 cell; Lane 6: HepG2 cell; Lane 7: Hek293 cell; Lane 8: NIH3T3 cell with ATG16L1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ATG16L1-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG16L1 monoclonal antibody, clone 5H9A11.Flow Cytometry
Flow cytometric analysis of HeLa cells with ATG16L1 monoclonal antibody (green) and negative control (red). -
Gene Info — ATG16L1
Entrez GeneID
55054Gene Name
ATG16L1
Gene Alias
APG16L, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30
Gene Description
ATG16 autophagy related 16-like 1 (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM
Other Designations
APG16 autophagy 16-like|APG16L beta|ATG16 autophagy related 16-like protein 1|WD repeat domain 30
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Interactome
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Disease
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