PRF1 monoclonal antibody, clone dG9
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More Files
- More Functions
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Specification
Product Description
Mouse monoclonal antibody raised against native human PRF1.
Immunogen
Native purified granules from human YT lymphoma cell line.
Host
Mouse
Reactivity
Bovine, Human
Specificity
This antibody recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Form
Liquid
Purification
Protein A purification
Isotype
IgG2b
Recommend Usage
Flow Cytometry
Immunoprecipitation
Immunohistochemistry (Frozen sections) (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 8.0 (15 mM sodium azide).
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Flow cytometry analysis (intracellular staining) of human peripheral blood cells with anti-human PRF1 PE. -
Gene Info — PRF1
Entrez GeneID
5551Gene Name
PRF1
Gene Alias
FLH2, HPLH2, MGC65093, P1, PFN1, PFP
Gene Description
perforin 1 (pore forming protein)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000019759|cytolysin|lymphocyte pore forming protein|perforin 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.
Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW.
PLoS Pathogens 2012 Nov; 8(11):e1002984.
Application:Flow Cyt, Human, Human PBMCs.
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A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities.
Diego A Vargas-Inchaustegui, Thorsten Demberg, and Marjorie Robert-Guroff.
Immunology 2011 Nov; 134(3):326.
Application:Flow Cyt, Monkey, NK cells.
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Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
Makoto Takeuchi, Toshiya Inoue, Takeshi Otani, Fumiyuki Yamasaki, Shuji Nakamura, Masayoshi Kibata.
Molecular and Cellular Biology 2010 Jun; 2(3):139.
Application:IF, Mouse, HOZOT cells.
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Flow cytometric detection of perforin upregulation in human CD8 T cells.
Adam R. Hersperger, George Makedonas, Michael R. Betts.
Cytometry. Part A : the Journal of the International Society for Analytical Cytology 2008 Nov; 73(11):1050.
Application:Flow Cyt, Human, PBMCs.
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Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.
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