TUBA1B monoclonal antibody, clone RM318
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TUBA1B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic acetylpeptide corresponding to residues surrounding Lys40 of human TUBA1B.
Reactivity
Human
Specificity
This antibody reacts to alpha-Tubulin acetylated at Lysine 40. No cross reactivity to non-acetylated alpha-Tubulin at Lysine 40.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:1000-5000)
Western Blot (1:1000-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) HeLa cells nontreated with Trichostatin A (TSA), (2) HeLa cells treated with Trichostatin A (TSA).Immunocytochemistry
Immunocytochemistry staining of Hela cells non-treated or treated with Trichostatin A (TSA) (red). Actin filaments labeled with fluorescein phalloidin (green) and nuclei with DAPI (blue). -
Gene Info — TUBA1B
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Interactome
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Pathway
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