HA-Tag chimeric monoclonal antibody, clone RMH02
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of 293T cells (1) transfected with a DNA construct encoding HA-Tag KRas fusion protein, (2) untransfected a DNA construct encoding HA-Tag KRas fusion protein.
Enzyme-linked Immunoabsorbent Assay
ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein.
ELISA analysis of chimeric human anti-HA-Tag antibody as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.
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Specification
Product Description
Human recombinant monoclonal antibody raised against recombinant HA-Tag.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to HA-tag (amino acids 98-106 of Human influenza hemagglutinin).
Specificity
This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (capturer: 0.5 ug/mL-2 ug/mL)
Detection Antibody (0.01 ug/mL-0.5 ug/mL)
Western Blot (0.1 ug/mL-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of 293T cells (1) transfected with a DNA construct encoding HA-Tag KRas fusion protein, (2) untransfected a DNA construct encoding HA-Tag KRas fusion protein.Enzyme-linked Immunoabsorbent Assay
ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein.
ELISA analysis of chimeric human anti-HA-Tag antibody as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.
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