BRAF V600E monoclonal antibody, clone RM8
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) cell lysates expressing endogenous mutant (V600E) BRAF, (2) wild type (WT) protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human WiDr cell line.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human thyroid carcinoma.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BRAF (V600E mutant).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human BRAF V600E mutant.
Reactivity
Human
Specificity
This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (2 ug/mL-10 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5 ug/mL-5 ug/mL)
Western Blot (0.5 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) cell lysates expressing endogenous mutant (V600E) BRAF, (2) wild type (WT) protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human WiDr cell line.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human thyroid carcinoma. -
Gene Info — BRAF
Entrez GeneID
673Gene Name
BRAF
Gene Alias
B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1
Gene Description
v-raf murine sarcoma viral oncogene homolog B1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
Other Designations
94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|Murine sarcoma viral (v-raf) oncogene homolog B1
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Interactome
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Pathway
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Disease
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Publication Reference
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Nodal histiocytic sarcoma with prominent eosinophilic infiltration: expression of eotaxin-2 on tumor cells.
Rintaro Ohe, Takanobu Kabasawa, Aya Utsunomiya, Yuka Urano, Takumi Kitaoka, Kazushi Suzuki, Naing Ye Aung, Ichiro Kawamura, Katsushi Tajima, Tomoharu Ishiyama, Mitsunori Yamakawa.
Diagnostic Pathology 2021 Jan; 16(1):6.
Application：IHC-P, Human, Human nodal histiocytic sarcoma.
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Nodal histiocytic sarcoma with prominent eosinophilic infiltration: expression of eotaxin-2 on tumor cells.
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