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Last updated: 2020/8/2

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H2AFX (phospho S139) monoclonal antibody, clone RM224 

  • Catalog # : MAB16908
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against human H2AFX (phospho S139).
  • Immunogen:
  • A phospho-peptide corresponding to phospho-histone H2AFX (S139).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.5 ug/mL-2 ug/mL)
    Immunocytochemistry (0.5 ug/mL-2 ug/mL)
    Multiplex (0.1 ug/mL-1 ug/mL)
    Western Blot (0.5 ug/mL-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of (1) acid extracts of HeLa cells treated with UV, (2) acid extracts of HeLa cells non-treated with UV.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of Hela cell line with antibody (red). Actin filaments labeled with fluorescein phalloidin (green).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3014
  • Gene Name:
  • H2AFX
  • Gene Alias:
  • H2A.X,H2A/X,H2AX
  • Gene Description:
  • H2A histone family, member X
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2AX histone
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