AOF1 monoclonal antibody, clone 5E10C1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: AOF1-hIgGFc transfected HEK293 cell with AOF1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with AOF1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with AOF1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AOF1 monoclonal antibody, clone 5E10C1.
Flow Cytometry
Flow cytometric analysis of Hela cells with AOF1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human AOF1.
Immunogen
Recombinant protein corresponding to amino acid 6-129 of human AOF1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
92
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: AOF1-hIgGFc transfected HEK293 cell with AOF1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with AOF1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with AOF1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AOF1 monoclonal antibody, clone 5E10C1.Flow Cytometry
Flow cytometric analysis of Hela cells with AOF1 monoclonal antibody (green) and negative control (red). -
Gene Info — AOF1
Entrez GeneID
221656Gene Name
AOF1
Gene Alias
C6orf193, DKFZp686I0412, FLJ33898, FLJ34109, FLJ43328, bA204B7.3, dJ298J15.2
Gene Description
amine oxidase (flavin containing) domain 1
Gene Ontology
HyperlinkGene Summary
flavin containing 1
Other Designations
OTTHUMP00000016075|OTTHUMP00000016077|OTTHUMP00000039336|amine oxidase, flavin containing 1
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Interactome
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+1-909-992-0619
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