ATF3 monoclonal antibody, clone 5A9B9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ATF3-hIgGFc transfected HEK293 cell with ATF3 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATF3 monoclonal antibody, clone 5A9B9.
Flow Cytometry
Flow cytometric analysis of A549 cells with ATF3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ATF3.
Immunogen
Recombinant protein corresponding to amino acid 1-181 of human ATF3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
20.6
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ATF3-hIgGFc transfected HEK293 cell with ATF3 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATF3 monoclonal antibody, clone 5A9B9.Flow Cytometry
Flow cytometric analysis of A549 cells with ATF3 monoclonal antibody (green) and negative control (red). -
Gene Info — ATF3
Entrez GeneID
467Gene Name
ATF3
Gene Alias
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Gene Description
activating transcription factor 3
Omim ID
603148Gene Ontology
HyperlinkGene Summary
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. [provided by RefSeq
Other Designations
ATF3deltaZip2|ATF3deltaZip2c|ATF3deltaZip3|OTTHUMP00000034887|OTTHUMP00000034890
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Interactome
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Disease
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