PAX6 monoclonal antibody, clone 1F6G2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PAX6-hIgGFc transfected HEK293 cell with PAX6 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with PAX6 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PAX6 monoclonal antibody, clone 1F6G2.
Flow Cytometry
Flow cytometric analysis of A549 cells with PAX6 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PAX6.
Immunogen
Recombinant protein corresponding to amino acid 1-122 of human PAX6 from E. coli.
Host
Mouse
Theoretical MW (kDa)
46.7
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PAX6-hIgGFc transfected HEK293 cell with PAX6 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with PAX6 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PAX6 monoclonal antibody, clone 1F6G2.Flow Cytometry
Flow cytometric analysis of A549 cells with PAX6 monoclonal antibody (green) and negative control (red). -
Gene Info — PAX6
Entrez GeneID
5080Gene Name
PAX6
Gene Alias
AN, AN2, D11S812E, MGC17209, MGDA, WAGR
Gene Description
paired box 6
Gene Ontology
HyperlinkGene Summary
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000038833|OTTHUMP00000038834|OTTHUMP00000038835|OTTHUMP00000038836|OTTHUMP00000038837|OTTHUMP00000038838|OTTHUMP00000038839|OTTHUMP00000038840|paired box gene 6|paired box homeotic gene-6
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Interactome
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Pathway
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Disease
- Abnormalities
- Alcoholism
- Atherosclerosis
- Atrophy
- Autistic Disorder
- Blepharoptosis
- Calcinosis
- Cleft Lip
- Cleft Palate
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