PROM1 monoclonal antibody, clone 2F8C5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PROM1-hIgGFc transfected HEK293 cell with PROM1 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PROM1 monoclonal antibody, clone 2F8C5.
Flow Cytometry
Flow cytometric analysis of Hela cells with PROM1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PROM1.
Immunogen
Recombinant protein corresponding to amino acid 20-108 of human PROM1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
97.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PROM1-hIgGFc transfected HEK293 cell with PROM1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PROM1 monoclonal antibody, clone 2F8C5.Flow Cytometry
Flow cytometric analysis of Hela cells with PROM1 monoclonal antibody (green) and negative control (red). -
Gene Info — PROM1
Entrez GeneID
8842Gene Name
PROM1
Gene Alias
AC133, CD133, MSTP061, PROML1, RP41
Gene Description
prominin 1
Omim ID
604365Gene Ontology
HyperlinkGene Summary
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
hProminin|hematopoietic stem cell antigen|prominin-like 1
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Interactome
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Disease
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