RAD18 monoclonal antibody, clone 2G8C10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RAD18-hIgGFc transfected HEK293 cell with RAD18 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD18 monoclonal antibody, clone 2G8C10.
Flow Cytometry
Flow cytometric analysis of Hela cells with RAD18 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAD18.
Immunogen
Recombinant protein corresponding to amino acid 332-430 of human RAD18 from E. coli.
Host
Mouse
Theoretical MW (kDa)
56.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RAD18-hIgGFc transfected HEK293 cell with RAD18 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD18 monoclonal antibody, clone 2G8C10.Flow Cytometry
Flow cytometric analysis of Hela cells with RAD18 monoclonal antibody (green) and negative control (red). -
Gene Info — RAD18
Entrez GeneID
56852Gene Name
RAD18
Gene Alias
RNF73
Gene Description
RAD18 homolog (S. cerevisiae)
Omim ID
605256Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq
Other Designations
RAD18, S. cerevisiae, homolog|postreplication repair protein hRAD18p
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Interactome
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Disease
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