GLI1 monoclonal antibody, clone 1B9F8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: GLI1-hIgGFc transfected HEK293 cell with GLI1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with GLI1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with GLI1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GLI1 monoclonal antibody, clone 1B9F8.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with GLI1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GLI1.
Immunogen
Recombinant protein corresponding to amino acid 284-449 of human GLI1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
118
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: GLI1-hIgGFc transfected HEK293 cell with GLI1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with GLI1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with GLI1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GLI1 monoclonal antibody, clone 1B9F8.Flow Cytometry
Flow cytometric analysis of HepG2 cells with GLI1 monoclonal antibody (green) and negative control (red). -
Gene Info — GLI1
Entrez GeneID
2735Gene Name
GLI1
Gene Alias
GLI
Gene Description
glioma-associated oncogene homolog 1 (zinc finger protein)
Omim ID
165220Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
glioma-associated oncogene homolog (zinc finger protein)|glioma-associated oncogene homolog 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Co-targeting FAK and Gli1 inhibits the tumor-associated macrophages-released CCL22-mediated esophageal squamous cell carcinoma malignancy.
Jie Chen, Yanmeng Zhu, Di Zhao, Lingyuan Zhang, Jing Zhang, Yuanfan Xiao, Qingnan Wu, Yan Wang, Qimin Zhan.
MedComm 2023 Oct; 4(6):e381.
Application：WB, IP, IHC, Human, KYSE410, KYSE510 (esophageal squamous cell carcinoma, ESCC cells).
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Co-targeting FAK and Gli1 inhibits the tumor-associated macrophages-released CCL22-mediated esophageal squamous cell carcinoma malignancy.
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