TARDBP monoclonal antibody, clone 7F9A6
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human TARDBP.
Immunogen
Recombinant protein corresponding to amino acid 126-260 of human TARDBP from E. coli.
Host
Mouse
Theoretical MW (kDa)
44.7
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: TARDBP-hIgGFc transfected HEK293 cell with TARDBP monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TARDBP monoclonal antibody, clone 7F9A6.Flow Cytometry
Flow cytometric analysis of Hela cells with TARDBP monoclonal antibody (green) and negative control (red). -
Gene Info — TARDBP
Entrez GeneID
23435Gene Name
TARDBP
Gene Alias
ALS10, TDP-43
Gene Description
TAR DNA binding protein
Omim ID
605078Gene Ontology
HyperlinkGene Summary
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq
Other Designations
OTTHUMP00000002171|TAR DNA-binding protein-43
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Interactome
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Disease
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