RAB11FIP1 monoclonal antibody, clone 3A12H9D2
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAB11FIP1.
Immunogen
Recombinant protein corresponding to amino acid 130-271 of human RAB11FIP1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
137.2
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Raji cell; Lane 2: A431 cell; Lane 3: HCT116 cell; Lane 4: LOVO cell; Lane 5: SW620 cell; Lane 6: SW480 cell; Lane 7: HepG2 cell and Lane 8: NIH/3T3 cell with RAB11FIP1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RAB11FIP1-hIgGFc transfected HEK293 cell with RAB11FIP1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with RAB11FIP1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAB11FIP1 monoclonal antibody, clone 3A12H9D2.Flow Cytometry
Flow cytometric analysis of Raji cells with RAB11FIP1 monoclonal antibody (green) and negative control (red). -
Gene Info — RAB11FIP1
Entrez GeneID
80223Gene Name
RAB11FIP1
Gene Alias
DKFZp686E2214, FLJ22524, FLJ22622, MGC78448, NOEL1A, RCP, rab11-FIP1
Gene Description
RAB11 family interacting protein 1 (class I)
Omim ID
608737Gene Ontology
HyperlinkGene Summary
Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP1 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]).[supplied by OMIM
Other Designations
RAB11 coupling protein|RAB11 family interacting protein 1|Rab effector protein|Rab-interacting recycling protein
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Interactome
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Pathway
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