CIDEC monoclonal antibody, clone 7C12F11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: A431 cell and Lane 3: HCT116 cell with CIDEC monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CIDEC-hIgGFc transfected HEK293 cell with CIDEC monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with CIDEC monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CIDEC monoclonal antibody, clone 7C12F11.
Flow Cytometry
Flow cytometric analysis of Hela cells with CIDEC monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CIDEC.
Immunogen
Recombinant protein corresponding to amino acid 53-141 of human CIDEC from E. coli.
Host
Mouse
Theoretical MW (kDa)
26.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: A431 cell and Lane 3: HCT116 cell with CIDEC monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CIDEC-hIgGFc transfected HEK293 cell with CIDEC monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with CIDEC monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CIDEC monoclonal antibody, clone 7C12F11.Flow Cytometry
Flow cytometric analysis of Hela cells with CIDEC monoclonal antibody (green) and negative control (red). -
Gene Info — CIDEC
Entrez GeneID
63924Gene Name
CIDEC
Gene Alias
CIDE-3, FLJ20871, Fsp27
Gene Description
cell death-inducing DFFA-like effector c
Gene Ontology
HyperlinkGene Summary
DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis. A novel family of cell death-inducing DFF45 (MIM 601882)-like effectors (CIDEs), including CIDEC, can also promote apoptosis (Liang et al., 2003 [PubMed 12429024]).[supplied by OMIM
Other Designations
cell death activator CIDE-3|fat specific protein 27
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Interactome
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