NOX4 monoclonal antibody, clone 3H2C4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: NOX4-hIgGFc transfected HEK293 cell with NOX4 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with NOX4 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with NOX4 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NOX4 monoclonal antibody, clone 3H2C4.
Flow Cytometry
Flow cytometric analysis of Hela cells with NOX4 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NOX4.
Immunogen
Recombinant protein corresponding to amino acid 210-310 of human NOX4 from E. coli.
Host
Mouse
Theoretical MW (kDa)
67
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: NOX4-hIgGFc transfected HEK293 cell with NOX4 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with NOX4 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with NOX4 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NOX4 monoclonal antibody, clone 3H2C4.Flow Cytometry
Flow cytometric analysis of Hela cells with NOX4 monoclonal antibody (green) and negative control (red). -
Gene Info — NOX4
Entrez GeneID
50507Gene Name
NOX4
Gene Alias
KOX, KOX-1, RENOX
Gene Description
NADPH oxidase 4
Omim ID
605261Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants
Other Designations
kidney superoxide-producing NADPH oxidase|renal NAD(P)H-oxidase
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Interactome
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Disease
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