EZR monoclonal antibody, clone 6F1A9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: Hela cell; Lane 3: A431 cell; Lane 4: Hek293 cell; Lane 5: SK-N-SH cell; Lane 6: Jurkat cell; Lane 7: HepG2 cell; Lane 8: NIH/3T3 cell and Lane 9: Cos7 cell with EZR monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: EZR-hIgGFc transfected HEK293 cell with EZR monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of A549 cells with EZR monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EZR monoclonal antibody, clone 6F1A9.
Flow Cytometry
Flow cytometric analysis of Hela cells with EZR monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human EZR.
Immunogen
Recombinant protein corresponding to amino acid 292-464 of human EZR from E. coli.
Host
Mouse
Theoretical MW (kDa)
69.4
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:100-1:400)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: Hela cell; Lane 3: A431 cell; Lane 4: Hek293 cell; Lane 5: SK-N-SH cell; Lane 6: Jurkat cell; Lane 7: HepG2 cell; Lane 8: NIH/3T3 cell and Lane 9: Cos7 cell with EZR monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: EZR-hIgGFc transfected HEK293 cell with EZR monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of A549 cells with EZR monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EZR monoclonal antibody, clone 6F1A9.Flow Cytometry
Flow cytometric analysis of Hela cells with EZR monoclonal antibody (green) and negative control (red). -
Gene Info — EZR
Entrez GeneID
7430Gene Name
EZR
Gene Alias
CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, VIL2
Gene Description
ezrin
Omim ID
123900Gene Ontology
HyperlinkGene Summary
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017506|OTTHUMP00000017507|cytovillin 2|villin 2 (ezrin)
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Interactome
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Pathway
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Disease
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