ANXA5 monoclonal antibody, clone 3B9B7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: PNAC-1 cell; Lane 3: NIH/3T3 cell; Lane 4: Hela cell; Lane 5: MCF-7 cell and Lane 6: A431 cell with ANXA5 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ANXA5-hIgGFc transfected HEK293 cell with ANXA5 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with ANXA5 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ANXA5 monoclonal antibody, clone 3B9B7.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with ANXA5 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ANXA5.
Immunogen
Recombinant protein corresponding to amino acid 160-320 of human ANXA5 from E. coli.
Host
Mouse
Theoretical MW (kDa)
36
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: PNAC-1 cell; Lane 3: NIH/3T3 cell; Lane 4: Hela cell; Lane 5: MCF-7 cell and Lane 6: A431 cell with ANXA5 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ANXA5-hIgGFc transfected HEK293 cell with ANXA5 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with ANXA5 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ANXA5 monoclonal antibody, clone 3B9B7.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with ANXA5 monoclonal antibody (green) and negative control (red). -
Gene Info — ANXA5
Entrez GeneID
308Gene Name
ANXA5
Gene Alias
ANX5, ENX2, PP4
Gene Description
annexin A5
Omim ID
131230Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq
Other Designations
anchorin CII|annexin 5|endonexin II|lipocortin V|placental anticoagulant protein I
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Interactome
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Disease
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