CSF3 monoclonal antibody, clone 8G5F7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CSF3-hIgGFc transfected HEK293 cell with CSF3 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of A549 cells with CSF3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CSF3 monoclonal antibody, clone 8G5F7.
Flow Cytometry
Flow cytometric analysis of A549 cells with CSF3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CSF3.
Immunogen
Recombinant protein corresponding to amino acid 1-207 of human CSF3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
22.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CSF3-hIgGFc transfected HEK293 cell with CSF3 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of A549 cells with CSF3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CSF3 monoclonal antibody, clone 8G5F7.Flow Cytometry
Flow cytometric analysis of A549 cells with CSF3 monoclonal antibody (green) and negative control (red). -
Gene Info — CSF3
Entrez GeneID
1440Gene Name
CSF3
Gene Alias
G-CSF, GCSF, MGC45931
Gene Description
colony stimulating factor 3 (granulocyte)
Omim ID
138970Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164327|colony stimulating factor 3|filgrastim|granulocyte colony stimulating factor|lenograstim|pluripoietin
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Interactome
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Pathway
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Disease
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