EMD monoclonal antibody, clone 8F5G2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: EMD-hIgGFc transfected HEK293 cell with EMD monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with EMD monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EMD monoclonal antibody, clone 8F5G2.
Flow Cytometry
Flow cytometric analysis of A549 cells with EMD monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human EMD.
Immunogen
Recombinant protein corresponding to amino acid 1-222 of human EMD from E. coli.
Host
Mouse
Theoretical MW (kDa)
29
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: EMD-hIgGFc transfected HEK293 cell with EMD monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with EMD monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EMD monoclonal antibody, clone 8F5G2.Flow Cytometry
Flow cytometric analysis of A549 cells with EMD monoclonal antibody (green) and negative control (red). -
Gene Info — EMD
Entrez GeneID
2010Gene Name
EMD
Gene Alias
EDMD, LEMD5, STA
Gene Description
emerin
Gene Ontology
HyperlinkGene Summary
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq
Other Designations
LEM domain containing 5|OTTHUMP00000031938|OTTHUMP00000061687
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Interactome
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Pathway
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Disease
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