WTAP monoclonal antibody, clone 6B6B6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: Hela cell; Lane 3: K562 cell; Lane 4: Hek293 cell; Lane 5: A549 cell; Lane 6: HepG2 cell; Lane 7: Jurkat cell and Lane 8: Cos7 cell with WTAP monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: WTAP-hIgGFc transfected HEK293 cell with WTAP monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with WTAP monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with WTAP monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WTAP monoclonal antibody, clone 6B6B6.
Flow Cytometry
Flow cytometric analysis of A549 cells with WTAP monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human WTAP.
Immunogen
Recombinant protein corresponding to amino acid 91-201 of human WTAP from E. coli.
Host
Mouse
Theoretical MW (kDa)
44.2
Reactivity
Human, Monkey
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: Hela cell; Lane 3: K562 cell; Lane 4: Hek293 cell; Lane 5: A549 cell; Lane 6: HepG2 cell; Lane 7: Jurkat cell and Lane 8: Cos7 cell with WTAP monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: WTAP-hIgGFc transfected HEK293 cell with WTAP monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with WTAP monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with WTAP monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WTAP monoclonal antibody, clone 6B6B6.Flow Cytometry
Flow cytometric analysis of A549 cells with WTAP monoclonal antibody (green) and negative control (red). -
Gene Info — WTAP
Entrez GeneID
9589Gene Name
WTAP
Gene Alias
DKFZp686F20131, KIAA0105, MGC3925
Gene Description
Wilms tumor 1 associated protein
Omim ID
605442Gene Ontology
HyperlinkGene Summary
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq
Other Designations
OTTHUMP00000017522|OTTHUMP00000017523|PNAS-132|WT1-associated protein|Wilms' tumour 1-associating protein|putative pre-mRNA splicing regulator female-lethal(2D)
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Interactome
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