ERCC1 monoclonal antibody, clone 1E5B3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ERCC1-hIgGFc transfected HEK293 cell with ERCC1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with ERCC1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ERCC1 monoclonal antibody, clone 1E5B3.
Flow Cytometry
Flow cytometric analysis of A549 cells with ERCC1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ERCC1.
Immunogen
Recombinant protein corresponding to amino acid 151-297 of human ERCC1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
32.6
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ERCC1-hIgGFc transfected HEK293 cell with ERCC1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with ERCC1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ERCC1 monoclonal antibody, clone 1E5B3.Flow Cytometry
Flow cytometric analysis of A549 cells with ERCC1 monoclonal antibody (green) and negative control (red). -
Gene Info — ERCC1
Entrez GeneID
2067Gene Name
ERCC1
Gene Alias
COFS4, UV20
Gene Description
excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
excision repair cross-complementing 1|excision repair protein
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Interactome
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Pathway
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Disease
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