GNAS monoclonal antibody, clone 7G6G5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: GNAS-hIgGFc transfected HEK293 cell with GNAS monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with GNAS monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GNAS monoclonal antibody, clone 7G6G5.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with GNAS monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GNAS.
Immunogen
Recombinant protein corresponding to amino acid 42-188 of human GNAS from E. coli.
Host
Mouse
Theoretical MW (kDa)
68
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: GNAS-hIgGFc transfected HEK293 cell with GNAS monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with GNAS monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GNAS monoclonal antibody, clone 7G6G5.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with GNAS monoclonal antibody (green) and negative control (red). -
Gene Info — GNAS
Entrez GeneID
2778Gene Name
GNAS
Gene Alias
AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, NESP, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3
Gene Description
GNAS complex locus
Gene Ontology
HyperlinkGene Summary
This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq
Other Designations
OTTHUMP00000031740|OTTHUMP00000031756|OTTHUMP00000196026|OTTHUMP00000196030|adenylate cyclase-stimulating G alpha protein|guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1|guanine nucleotide regulatory protein|neuroe
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Interactome
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Pathway
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Disease
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