ATG5 monoclonal antibody, clone 8E8G6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela celland Lane 2: K562 cell with ATG5 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with ATG5 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG5 monoclonal antibody, clone 8E8G6.
Flow Cytometry
Flow cytometric analysis of Hela cells with ATG5 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human ATG5.
Immunogen
A synthetic peptide corresponding to amino acid of human ATG5.
Host
Mouse
Theoretical MW (kDa)
32.4
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela celland Lane 2: K562 cell with ATG5 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with ATG5 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG5 monoclonal antibody, clone 8E8G6.Flow Cytometry
Flow cytometric analysis of Hela cells with ATG5 monoclonal antibody (green) and negative control (red). -
Gene Info — ATG5
Entrez GeneID
9474Gene Name
ATG5
Gene Alias
APG5, APG5-LIKE, APG5L, ASP, hAPG5
Gene Description
ATG5 autophagy related 5 homolog (S. cerevisiae)
Omim ID
604261Gene Ontology
HyperlinkGene Summary
S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
Other Designations
APG5 (autophagy 5, S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
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Interactome
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Pathway
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Disease
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