ADD1 monoclonal antibody, clone 5D4H1
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ADD1.
Immunogen
Recombinant protein corresponding to amino acid 1-193 of human ADD1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
81
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ADD1-hIgGFc transfected HEK293 cell with ADD1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with ADD1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ADD1 monoclonal antibody, clone 5D4H1. -
Gene Info — ADD1
Entrez GeneID
118Gene Name
ADD1
Gene Alias
ADDA, MGC3339, MGC44427
Gene Description
adducin 1 (alpha)
Gene Ontology
HyperlinkGene Summary
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq
Other Designations
OTTHUMP00000151164|OTTHUMP00000151165|erythrocyte adducin alpha subunit
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