TUBB1 monoclonal antibody, clone 2A1A9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: HepG2; Lane 3: A431 cell; Lane 4: Jurkat cell; Lane 5: Hela; Lane 6: NIH/3T3 cell; Lane 7: Cos7 cell and Lane 8: PC-12 cell with TUBB1 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: TUBB1-hIgGFc transfected HEK293 cell with TUBB1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with TUBB1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with TUBB1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TUBB1 monoclonal antibody, clone 2A1A9.
Flow Cytometry
Flow cytometric analysis of A431 cells with TUBB1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human TUBB1.
Immunogen
Recombinant protein corresponding to amino acid 33-166 of human TUBB1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
50.3
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: HepG2; Lane 3: A431 cell; Lane 4: Jurkat cell; Lane 5: Hela; Lane 6: NIH/3T3 cell; Lane 7: Cos7 cell and Lane 8: PC-12 cell with TUBB1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: TUBB1-hIgGFc transfected HEK293 cell with TUBB1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with TUBB1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with TUBB1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TUBB1 monoclonal antibody, clone 2A1A9.Flow Cytometry
Flow cytometric analysis of A431 cells with TUBB1 monoclonal antibody (green) and negative control (red). -
Gene Info — TUBB1
Entrez GeneID
81027Gene Name
TUBB1
Gene Alias
dJ543J19.4
Gene Description
tubulin, beta 1
Gene Ontology
HyperlinkGene Summary
Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM
Other Designations
OTTHUMP00000031411|beta tubulin 1, class VI
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Interactome
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Pathway
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Disease
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