ERBB4 monoclonal antibody, clone 5G6B4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ERBB4-hIgGFc transfected HEK293 cell with ERBB4 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with ERBB4 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ERBB4 monoclonal antibody, clone 5G6B4.
Flow Cytometry
Flow cytometric analysis of Hela cells with ERBB4 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ERBB4.
Immunogen
Recombinant protein corresponding to amino acid 1159-1308 of human ERBB4 from E. coli.
Host
Mouse
Theoretical MW (kDa)
146.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: ERBB4-hIgGFc transfected HEK293 cell with ERBB4 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with ERBB4 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ERBB4 monoclonal antibody, clone 5G6B4.Flow Cytometry
Flow cytometric analysis of Hela cells with ERBB4 monoclonal antibody (green) and negative control (red). -
Gene Info — ERBB4
Entrez GeneID
2066Gene Name
ERBB4
Gene Alias
HER4, MGC138404, p180erbB4
Gene Description
v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Omim ID
600543Gene Ontology
HyperlinkGene Summary
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4|receptor tyrosine-protein kinase erbB-4|tyrosine kinase-type cell surface receptor HER4|v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4|v-erb-a erythroblastic leukemia v
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Interactome
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Pathway
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Disease
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