EZH2 monoclonal antibody, clone 6G4F4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: EZH2-hIgGFc transfected HEK293 cell with EZH2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with EZH2 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EZH2 monoclonal antibody, clone 6G4F4.
Flow Cytometry
Flow cytometric analysis of Hela cells with EZH2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human EZH2.
Immunogen
Recombinant protein corresponding to amino acid 1-194 of human EZH2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
85.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: EZH2-hIgGFc transfected HEK293 cell with EZH2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with EZH2 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EZH2 monoclonal antibody, clone 6G4F4.Flow Cytometry
Flow cytometric analysis of Hela cells with EZH2 monoclonal antibody (green) and negative control (red). -
Gene Info — EZH2
Entrez GeneID
2146Gene Name
EZH2
Gene Alias
ENX-1, EZH1, KMT6, MGC9169
Gene Description
enhancer of zeste homolog 2 (Drosophila)
Omim ID
601573Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
enhancer of zeste 2
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Interactome
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Disease
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