SSH1 monoclonal antibody, clone 1A5C8
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human SSH1.
Immunogen
A synthetic peptide corresponding to amino acid 1032-1044 of human SSH1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
115.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunocytochemical staining of Hela cells with SSH1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SSH1 monoclonal antibody, clone 1A5C8.Flow Cytometry
Flow cytometric analysis of Hela cells with SSH1 monoclonal antibody (green) and negative control (red). -
Gene Info — SSH1
Entrez GeneID
54434Gene Name
SSH1
Gene Alias
FLJ38102, KIAA1298, SSH-1
Gene Description
slingshot homolog 1 (Drosophila)
Omim ID
606778Gene Ontology
HyperlinkGene Summary
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM
Other Designations
slingshot 1|slingshot homolog 1
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Interactome
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