HIST2H3A monoclonal antibody, clone 6D3B9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: C6 cell; Lane 3: HEK293 cell; Lane 4: PC-12 cell and Lane 5: NIH/3T3cell with HIST2H3A monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIST2H3A monoclonal antibody, clone 6D3B9.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with HIST2H3A monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human HIST2H3A.
Immunogen
A synthetic peptide corresponding to amino acid 121-136 of human HIST2H3A from E. coli.
Host
Mouse
Theoretical MW (kDa)
15.4
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: C6 cell; Lane 3: HEK293 cell; Lane 4: PC-12 cell and Lane 5: NIH/3T3cell with HIST2H3A monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIST2H3A monoclonal antibody, clone 6D3B9.Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with HIST2H3A monoclonal antibody (green) and negative control (red). -
Gene Info — HIST2H3A
Entrez GeneID
333932Gene Name
HIST2H3A
Gene Alias
H3/n, H3/o
Gene Description
histone cluster 2, H3a
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq
Other Designations
histone 2, H3a|histone H3/o
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Interactome
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Pathway
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