HIST3H3 monoclonal antibody, clone 4E9B11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH/3T3 cell; Lane 2: Hela cell and Lane 3: K562 cell with HIST3H3 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIST3H3 monoclonal antibody, clone 4E9B11.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with HIST3H3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human HIST3H3.
Immunogen
A synthetic peptide corresponding to amino acid of human HIST3H3.
Host
Mouse
Theoretical MW (kDa)
15.5
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH/3T3 cell; Lane 2: Hela cell and Lane 3: K562 cell with HIST3H3 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HIST3H3 monoclonal antibody, clone 4E9B11.Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with HIST3H3 monoclonal antibody (green) and negative control (red). -
Gene Info — HIST3H3
Entrez GeneID
8290Gene Name
HIST3H3
Gene Alias
H3.4, H3/g, H3FT, H3t, MGC126886, MGC126888
Gene Description
histone cluster 3, H3
Omim ID
602820Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member T|OTTHUMP00000037945|histone 3, H3
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Interactome
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Pathway
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