SNAI2 monoclonal antibody, clone 4B6D5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using SNAI2 monoclonal antibody against MCF-7 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: SNAI2-hIgGFc transfected HEK293 cell with SNAI2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with SNAI2 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with SNAI2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SNAI2 monoclonal antibody, clone 4B6D5.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with SNAI2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SNAI2.
Immunogen
Recombinant protein corresponding to amino acid 100-200 of human SNAI2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
30
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using SNAI2 monoclonal antibody against MCF-7 cell lysate.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: SNAI2-hIgGFc transfected HEK293 cell with SNAI2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with SNAI2 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with SNAI2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SNAI2 monoclonal antibody, clone 4B6D5.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with SNAI2 monoclonal antibody (green) and negative control (red). -
Gene Info — SNAI2
Entrez GeneID
6591Gene Name
SNAI2
Gene Alias
MGC10182, SLUG, SLUGH1, WS2D
Gene Description
snail homolog 2 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq
Other Designations
OTTHUMP00000195093|neural crest transcription factor SLUG|slug (chicken homolog), zinc finger protein|slug homolog, zinc finger protein|snail 2
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Interactome
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Pathway
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Disease
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