CASP7 monoclonal antibody, clone 4D10B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell; Lane 2: HEK293 cell; Lane 3: MOLT4 cell; Lane 4: MCF-7 cell and Lane 5: PC-12 cell with CASP7 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CASP7-hIgGFc transfected HEK293 cell with CASP7 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with CASP7 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CASP7 monoclonal antibody, clone 4D10B2.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with CASP7 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CASP7.
Immunogen
Recombinant protein corresponding to amino acid 29-198 of human CASP7 from E. coli.
Host
Mouse
Theoretical MW (kDa)
34.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell; Lane 2: HEK293 cell; Lane 3: MOLT4 cell; Lane 4: MCF-7 cell and Lane 5: PC-12 cell with CASP7 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CASP7-hIgGFc transfected HEK293 cell with CASP7 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with CASP7 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CASP7 monoclonal antibody, clone 4D10B2.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with CASP7 monoclonal antibody (green) and negative control (red). -
Gene Info — CASP7
Entrez GeneID
840Gene Name
CASP7
Gene Alias
CMH-1, ICE-LAP3, MCH3
Gene Description
caspase 7, apoptosis-related cysteine peptidase
Omim ID
601761Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 3|Lice2 alpha/beta/gamma|OTTHUMP00000020510|OTTHUMP00000020511|OTTHUMP00000020512|OTTHUMP00000020514|apoptotic protease MCH-3|caspase 7|caspase 7 isoform delta|caspase 7, apoptosis-related cysteine protease
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Interactome
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Pathway
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Disease
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