WAS monoclonal antibody, clone 7B10E4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: WAS-hIgGFc transfected HEK293 cell with WAS monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with WAS monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WAS monoclonal antibody, clone 7B10E4.
Flow Cytometry
Flow cytometric analysis of Hela cells with WAS monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human WAS.
Immunogen
Recombinant protein corresponding to amino acid 57-170 of human WAS from E. coli.
Host
Mouse
Theoretical MW (kDa)
53
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: WAS-hIgGFc transfected HEK293 cell with WAS monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with WAS monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WAS monoclonal antibody, clone 7B10E4.Flow Cytometry
Flow cytometric analysis of Hela cells with WAS monoclonal antibody (green) and negative control (red). -
Gene Info — WAS
Entrez GeneID
7454Gene Name
WAS
Gene Alias
IMD2, THC, WASP
Gene Description
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Gene Ontology
HyperlinkGene Summary
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq
Other Designations
OTTHUMP00000032395|Wiskott-Aldrich syndrome protein|thrombocytopenia 1 (X-linked)
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Interactome
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Pathway
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Disease
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