CCNA2 monoclonal antibody, clone 6B4D11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: HEK293 cell; Lane 3: Jurkat cell; Lane 4: K562 cell; Lane 5: SK-Br-3 cell and Lane 6: NIH/3T3 cell with CCNA2 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CCNA2-hIgGFc transfected HEK293 cell with CCNA2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with CCNA2 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CCNA2 monoclonal antibody, clone 6B4D11.
Flow Cytometry
Flow cytometric analysis of A431 cells with CCNA2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CCNA2.
Immunogen
Recombinant protein corresponding to amino acid 105-233 of human CCNA2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
48.6
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: HEK293 cell; Lane 3: Jurkat cell; Lane 4: K562 cell; Lane 5: SK-Br-3 cell and Lane 6: NIH/3T3 cell with CCNA2 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CCNA2-hIgGFc transfected HEK293 cell with CCNA2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with CCNA2 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CCNA2 monoclonal antibody, clone 6B4D11.Flow Cytometry
Flow cytometric analysis of A431 cells with CCNA2 monoclonal antibody (green) and negative control (red). -
Gene Info — CCNA2
Entrez GeneID
890Gene Name
CCNA2
Gene Alias
CCN1, CCNA
Gene Description
cyclin A2
Omim ID
123835Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq
Other Designations
cyclin A
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Interactome
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Pathway
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Disease
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