WT1 monoclonal antibody, clone 5G11A5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: WT1-hIgGFc transfected HEK293 cell with WT1 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WT1 monoclonal antibody, clone 5G11A5.
Flow Cytometry
Flow cytometric analysis of Hela cells with WT1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human WT1.
Immunogen
Recombinant protein corresponding to amino acid 314-479 of human WT1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
49.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: WT1-hIgGFc transfected HEK293 cell with WT1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of WT1 monoclonal antibody, clone 5G11A5.Flow Cytometry
Flow cytometric analysis of Hela cells with WT1 monoclonal antibody (green) and negative control (red). -
Gene Info — WT1
Entrez GeneID
7490Gene Name
WT1
Gene Alias
GUD, WAGR, WIT-2, WT33
Gene Description
Wilms tumor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
Other Designations
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Interactome
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Disease
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